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KMID : 0613820130230101246
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Journal of Life Science
2013 Volume.23 No. 10 p.1246 ~ p.1251
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Organic Solvent-tolerant Lipase from Pseudomonas sp. BCNU 154
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Choi Hye-Jung
Hwang Min-Jung
Seo Jeoung-Yoon
Joo Woo-Hong
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Abstract
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An organic solvent-tolerant lipase of Pseudomonas sp. BCNU 154 that was isolated from wastewater in the industrial complex region had optimal activity at 37℃ and pH 8. This crude extracellular lipase from BCNU 154 exhibited maximum stability in toluene, retaining about 6.01 U/ml (117.53%) activity for 2 h. Ca²+,Mg²+,NH₄+, and Na+ ions and triton X-100 activated the enzymes, whereas Ba²+,Hg²+, and Zn²+ ions inhibited their activity. Pseudomonas sp. BCNU 154 lipase revealed stable activity comparable to that of the commercial immobilized Novozym 435. Thus, this organic solvent-tolerant lipase could have potential as a whole cell biocatalyst in industrial chemical processes without the use of immobilization.
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KEYWORD
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Lipase stability, organic solvent tolerant lipase, Pseudomonas sp., Pseudomonas sp. BCNU 154
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